Publications

Research Articles 

2023

1.  Santana DJ, Anku JAE, Zhao G, Zarnowski R, Johnson CJ, Hautau H, Visser ND, Ibrahim AS, Andes D, Nett JE, Singh S*, O'Meara TR. A Candida auris-specific adhesin, Scf1, governs surface association, colonization, and virulence. Science. 2023 Sep 29;381(6665):1461-1467. Epub 2023 Sep 28. PMID: 37769084.

2. Phan QT, Solis NV, Cravener MV, Swidergall M, Lin J, Huang MY, Liu H, Singh S, Ibrahim AS, Mazzone M, Mitchell AP, Filler SG. Candida albicans stimulates formation of a multi-receptor complex that mediates epithelial cell invasion during oropharyngeal infection. PLoS Pathogens. 2023 Aug 23;19(8):e1011579. PMID: 37611070.

3. Singh S, Barbarino A, Youssef EG, Coleman D, Gebremariam T, Ibrahim AS. Protective Efficacy of Anti-Hyr1p Monoclonal Antibody against Systemic Candidiasis Due to Multi-Drug-Resistant Candida auris. Journal of Fungi. 2023 Jan 12;9(1):103. PMID: 36675924.

2022

4. Sutar Y, Nabeela S, Singh S, Alqarihi A, Solis N, Filler S, Ghebremariam T, Ibrahim AS, Date A, Uppuluri P.  Niclosamide loaded Eudragit EPO nanoparticles show enhanced Candida biofilm penetration, trigger biofilm detachment and protect from mucosal candidiasis. PLoS Biology. 2022 Aug 17;20(8):e3001762. PMID: 35976859.

5. Singh S, Nabeela S, Barbarino A, Ibrahim AS, Uppuluri P. Antibodies targeting Candida albicans Als3 and Hyr1 antigens protect neonatal mice from candidiasis. Frontiers in Immunology. 2022 Jul 22;13:925821. PMID: 35935947.

6. Phan QT, Solis NV, Lin J, Swidergall M, Singh S, Liu H, Sheppard DC, Ibrahim AS, Mitchell AP, Filler SG. Identification of Serum Bridging Molecules that Mediate Human Endothelial Cell Invasion by Candida species. PLoS Pathogens. 2022 Jul 7;18(7):e1010681. PMID: 35797411.

2021

7. Gebremariam T, Gu Y, Singh S, Kitt TM, Ibrahim AS. Combination treatment of liposomal amphotericin B and isavuconazole is synergistic in treating experimental mucormycosis. Journal of Antimicrobial Chemotherapy. 2021 Jul 15. PMID: 34263306. 

8. Ost K, O’ Meara TR, Stephens WZ, Chiaro T, Zhou H, Penman J, Bell R, Catanzaro JR, Song D, Singh S, Call DH, Hwang-Wong E, Hanson KE, Valentine JF, Christensen KA, O’Connell RM, Cormack B, Ibrahim AI, Palm NW, Noble SM & Round JL. Adaptive immunity promotes a mutually beneficial interaction between commensal eukaryotes. Nature. 2021 July 14, 596, pages114–118. PMID: 34262174. 

9. Mamouei Z, Singh S, Lemire B, Gu Y, Alqarihi A, Nabeela S, Li D, Ibrahim AS, Uppuluri P. An evolutionarily diverged mitochondrial protein controls biofilm growth and virulence in Candida albicans. PloS Biology. 2021 March 15. PMID: 33720927.  

10. Soliman SSM*, Baldin C*, Gu Y*, Singh S*, Gebremariam T, Swidergall M, Alqarihi A, Youssef EG, Alkhazraji S, Pikoulas A, Perske C, Venkataramani V, Rich A, Bruno VM, Hotopp JD, Mantis NJ, Edwards JE Jr, Filler SG, Chamilos G, Vitetta ES, Ibrahim AS. Mucoricin is a Ricin-Like Toxin that is Critical for the Pathogenesis of Mucormycosis. Nature Microbiology. 2021 Jan 18. PMID: 33462434. (*Equal contribution) 

2020

11. Iyer KA, Camara K, Daniel-Ivadd M, Trilles R, Pimentel-Elardod SM, Fossen JL, Marchillo K, Liu Z, Singh S, Muñoz JF, Kim SH, Porco JA Jr, Cuomo CA, Williams NS, Ibrahim AS, Edwards JE Jr, Andes DR, Nodwell JR, Brown LE, Whitesell L, Robbins N, Cowen LE. Novel 3,3-diarylated oxindole efflux inhibitor provides a promising approach to combat azole resistance in the emerging fungal pathogen Candida auris. Nature Communications. 2020 Dec 22; 11, 6429. PMID: 33353950. 

12. Singh S, Yanow S and Agrawal B. Editorial: Heterologous Immunity: Implications and Applications in Vaccines and Immunotherapies. Frontiers in Immunology. 2020 July 7; 11:1408. PMID: 32774334. (Editorial) 

13. Youssef EG, Zhang L, Alkhazraji S, Ghebremariam T, Singh S, Yount NY, Yeaman MR, Uppuluri P, Ibrahim AS. Monoclonal IgM antibodies raised against Candida albicans Hyr1 provide cross-kingdom protection against Gram negative bacteria. Frontiers in Immunology. 2020 Feb 18; 11:76. PMID: 32153560. 

2019 

14. Alkhazraji S, Gebremariam T, Alqarihi A, Gu Y, Mamouei Z, Singh S, Wiederhold N, Shaw KJ and Ibrahim AS. Fosmanogepix (APX001) is Effective in the treatment of immunocompromised mice infected with invasive pulmonary Scedosporiosis or disseminated Fusariosis. Antimicrobial Agents and Chemotherapy. 2019 Dec 9; PMID: 31818813. 

15. Gebremariam T, Alkhazraji S, Gu Y, Singh S, Alqarihi A, Shaw KJ, Ibrahim A. Galactomannan is a Biomarker of Fosmanogepix (APX001) Efficacy in Treating Experimental Invasive Pulmonary Aspergillosis. Antimicrobial Agents and Chemotherapy. 2019 Nov 4; AAC.01966-19. PMID: 31685475. 

16. Singh S, Uppuluri P, Mamouei Z, Alqarihi A, Elhassan H, French S, Lockhart SR, Chiller T, Edwards Jr JE, Ibrahim AS. The NDV-3A vaccine protects mice from multidrug resistant Candida auris infection. PLoS Pathogens. 2019 Aug 5; 15(8): e1007460. PMID: 31381597. 

17. Alqarihi A*, Singh S*, Edwards Jr JE, Ibrahim AS, Uppuluri P. NDV-3A vaccination protects mice from Candida albicans biofilm infection. Nature Scientific Report. 2019 Apr 17;9(1):6194. PMID: 30996274. (*Equal contribution). 

18. Agrawal B, Gupta N, Vedi S, Singh S, Li W, Garg S, Li J, Kumar R. Heterologous immunity between adenoviruses and hepatitis C virus (HCV): Recombinant adenovirus vaccine vectors containing antigens from unrelated pathogens induce cross-reactive immunity against HCV antigens. Cells. 2019 May 26;8(5): 507. PMID: 31130710. 

2018

19. Mamouei Z, Alqarihi A, Singh S, Shuying Xu, Michael K. Mansour, Ibrahim AS, and Uppuluri P. Alexidine dihydrochloride has broad spectrum activities against diverse fungal pathogens. mSphere. 2018 Oct 31;3(5). PMID: 30381356. 

20. Uppuluri P, Singh S, Alqarihi A, Alqarihi A, Schmidt CS, Hennessey JP, Yeaman MR, Filler SG, Edwards JE, and Ibrahim AS. Human Als3p Antibodies are Surrogate Markers of NDV-3A Vaccine Efficacy Against Recurrent Vulvovaginal Candidiasis. Frontiers in Immunology. 2018 Jun 15; 9:1349. PMID: 29963049. 

21. Singh S, Ramirez E, Doueiri R, Valentin A, Rosati M, Hu X, Keele BF, Shen X, Tomaras GD, Ferrari G, LaBranche C, Montefiori DC, Das J, Alter G, Trinh HV, Hamlin C, Rao M, Dayton F, Bear J, Chowdhury B, Alicea C, Lifson JD, Broderick KE, Sardesai NY, Sivananthan SJ, Fox CB, Reed SG, Venzon DJ, Hirsch VM, Pavlakis GN, Felber BK. DNA and protein co-immunization regimens combined with different Toll-like receptor-4 (TLR-4) based adjuvants induce immune responses controlling SIVsmE660 infection. Journal of Virology. 2018 Jul 17; 92(15). PMID: 29793957. 

2017

22. Agrawal B, Singh S, Gupta N, Li W, Vedi S,  and R. Kumar. Unsolved Puzzles Surrounding HCV Immunity: Heterologous Immunity Adds Another Dimension. International Journal of Molecular Science. 2017 Jul 27, 18(8):1626. PMID: 28749434. (Review Article)

2016

23. Singh S, Vedi S, Samrat SK, Li W, Kumar R, and Agrawal B. Heterologous Immunity Between Adenoviruses and Hepatitis C Virus: A New Paradigm in HCV Immunity and Vaccines. PLoS One.  2016 Jan 11; 11(1): e0146404. PMID: 26751211. 

2015

24. Kumar A*, and Singh S*. Editorial: Influenza Virus Vaccines and Immunotherapies. Frontiers in Immunology. 2015 Nov 09; 6:599. PMID: 26635813. (*Equal and Corresponding author, Editorial). 

25. Samrat SK, Vedi S, Li W, Singh S, Samrat SK, Kumar R, and Agrawal B. Immunization with recombinant adenoviral vectors expressing HCV core or F proteins leads to T cells with reduced effector molecules granzyme B and IFN-γ: A potential new strategy for immune evasion in HCV infection. Viral Immunology. 2015 Jul 2; 28(6): 309-24. PMID: 26133045. 

2014

26. Singh S, Vedi S, Li W, Samrat SK, Kumar R, Agrawal B. Recombinant Adenoviral Vector expressing HCV NS4 Induce Protective Immune Responses in A Mouse Model of Vaccinia-HCV Virus Infection: A Dose and Route Conundrum. Vaccine. 2014 May 13;32(23):2712-21. PMID: 24631092. 

27. Appaiahgari MB, Glass R, Singh S*, Taneja S, Chandola TR, Bhandari N, Mishra S, and Vrati S. Transplacental rotavirus IgG interferes with immune response to live oral rotavirus vaccine ORV-116E in Indian infants. Vaccine. 2014 Feb 3; 32(6): 651-6. PMID: 24374502. 

28. Samrat SK, Li W, Singh S, Kumar R, and Agrawal B. Alternate reading frame protein (F protein) of hepatitis C virus: Paradoxical effects of activation and apoptosis on human dendritic cells lead to stimulation of T cells. Plos One.  2014 Jan 27; 9(1): e86567. PMID: 24475147. 

2013

29. Chandola TR, Taneja S, Goyal N, Rathore SS, Appaiahgari MB, Mishra A, Singh S*, and Vrati S* and Bhandari N. Descriptive epidemiology of rotavirus infection in a community in North India. Epidemiology and Infection.  2013 Oct; 141(10): 2094-100. PMID: 23298643. 

2009

30. Bhandari N, Sharma P, Taneja S, Kumar T, Rongsen-Chandola T, Appaiahgari MB, Mishra A, Singh S*, Vrati S*, and Rotavirus Vaccine Development Group. A dose-escalation safety and immunogenicity study of live attenuated oral rotavirus vaccine 116E in infants: a randomized, double-blind, placebo-controlled trial. Journal of Infectious Disease. 2009 Aug 1; 200(3): 421-9. PMID: 19545211. 

Abstracts  (Peer reviewed)

2023

1. McSwiggin H, Magalhaes R, Singh S, Nabeela S, Mattos E, Barbarino A, Yan W, Ibrahim AS, Uppuluri P. A Spatially Resolved Transcriptomic "Atlas" of Oropharyngeal Candidiasis Reveals A Th-2 and Th-17 Response, Similar to Allergic Inflammation. GRC Immunology of Fungal Infections; 2023 Jan 22-27; Galveston, TX, USA.

2. Barbarino A, Singh S, Youssef E, Nabeela S, Gebremariam T, Alkhazraji S, Ibrahim AS. Beta-Glucan Chitosan Particle Provides Cross-protection against Multi-drug Resistant Candida auris. GRC Immunology of Fungal Infections; 2023 Jan 22-27; Galveston, TX, USA 

3. Singh S, Barbarino A, Youssef E, Nabeela S, Gebremariam T, Alkhazraji S, Garry Osteroff, Uppuluri P, Edwards JE, Ibragim AS. Next Generation Als3p/Hyr1p Dual Antigen Vaccine Targeting Multi-Drug Resistant Fungal and Bacterial Infection. GRC Immunology of Fungal Infections; 2023 Jan 22-27; Galveston, TX, USA.

2022

4. Singh S, Nabeela S, Barbarino A, Ibrahim AS, Uppuluri P. 574. Antibodies Targeting Candida albicans Als3 and Hyr1 Antigens Protect Neonatal Mice from Candidiasis, Open Forum Infectious Diseases, Volume 9, Issue Supplement_2, December 2022, ofac492.627.

5. Singh S, Nabeela S, Barbarino A, Ibrahim AS, Uppuluri P. Antibodies Targeting Candida albicans Als3 and Hyr1 Antigens Protect Neonatal Mice from Candidiasis. IDWeek; 2022 Oct 19-21, Washington DC, USA 

6. Singh S, Barbarino A, Youssef E, Nabeela S, Teklegiorgis G, Ibrahim AS. 97. A Cross-kingdom Vaccine Protects against Multiple Healthcare-associated Infections, Open Forum Infectious Diseases, Volume 9, Issue Supplement_2, December 2022, ofac492.175.

7. Singh S, Barbarino A, Youssef E, Nabeela S, Teklegiorgis G, Ibrahim AS. A Cross-Kingdom Vaccine Protects against Multiple Healthcare-associated Infections. IDWeek; 2022 Oct 19-21, Washington DC, USA  

8. Youseff E, Singh S, Alkhazraji S, Gebremariam T, Ibrahim AS. 1705. Filamentous Hemagglutinin Polyclonal Antibodies Protect against Multidrug resistant Gram-negative bacteria, Open Forum Infectious Diseases, Volume 9, Issue Supplement_2, December 2022, ofac492.1335.

9. Youseff E, Singh S, Alkhazraji S, Gebremariam T, Ibrahim AS. Polyclonal antibodies against Filamentous hemagglutinin protect against multi-drug resistant Gram-negative bacteria. IDWeek; 2022 Oct 19-21, Washington DC, USA.

2021

10.  Gu Y, Alqarihi A, Singh S, Gebremariam T, Alkhazraji S, Youssef E, Andes D, Ibrahim AS. A Humanized Antibody Targeting the CotH Invasins is Protective Against Murine Mucormycosis. IDWeek; 2021 Sept 29-Oct 3, Online conference.

11. Gu Y, Alqarihi A, Singh S, Gebremariam T, Alkhazraji S, Youssef E, Andes D, Ibrahim AS. A Humanized Antibody Targeting the CotH Invasins is Protective Against Murine Mucormycosis, Open Forum Infectious Diseases, Volume 8, Issue Supplement_1, November 2021, Pages S71–S72.

12.  Mamouei Z, Singh S, Alquirihi A, Uppuluri P. Evolutionarily acquired insertion sequences in the Candida proteome are required for adaptation and virulence. Candida and Candidiasis; 2021 Mar 21-27; Online, Microbiology Society.

2020

13. Gebremariam T, Gu Y, Singh S, Kitt T, Ibrahim AS. Combination treatment of liposomal amphotericin B and isavuconazole is synergistic in treating experimental mucormycosis. ID Week; 2020 October 21-25; Philadelphia, PA, USA.

14. Gebremariam T, Gu Y, Singh S, Kitt T, Ibrahim AS. 745. Combination Treatment of Liposomal Amphotericin B and Isavuconazole is Synergistic in Treating Experimental Mucormycosis, Open Forum Infectious Diseases, Volume 7, Issue Supplement_1, October 2020, Page S420.

15. Soliman SSM, Baldin C, Gu Y, Singh S, Gebremariam T, Swidergall M, Alqarihi A, Youssef EG, Alkhazraji S, Pikoulas A, Perske C, Venkataramani V, Rich A, Bruno VM, Hotopp JD, Mantis NJ, Edwards JE Jr, Filler SG, Chamilos G, Vitetta ES, Ibrahim AS. Mucoricin is a Ricin-Like Toxin that is Critical for the Pathogenesis of Mucormycosis. 9th Advances Against Aspergillosis and Mucormycosis. 2020 Feb 27-29, Lugano, Switzerland.

16. Agrawal B, Singh S, Gupta N., Vedi S, Li W, Garg S, Li J, Kumar R. Heterologous Immunity and Hepatitis C Virus: Impact on Natural Infection, Pathogenesis and Vaccine Design. Proceedings 2020, 50, 63.

2019

17. Singh S, Heewon J, Alkhazraji S, Rich A, Ahmed A, Ruback S, Munford R, Vitetta ES, Ibrahim AS. Rhizopus delemar produces a ricin-like toxin that is involved in its pathogenicity. GRC Immunology of Fungal Infections. 2019 Jan 13-18; Galveston, TX. 

18. Singh S, Uppuluri P, Alqarihi A, Elhassan H, French S, Lockhart SR, Chiller T, Edwards JE and Ibrahim AS. Candida auris possesses homologs of Candida albicans Als3, and can be targeted by the vaccine NDV-3A. GRC Immunology of Fungal Infections. 2019 Jan 13-18; Galveston, TX.

19. Mamouei Z, Singh S, Alqarihi A, Zeng G, Wang Y, Edwards JE, Ibrahim AS, Uppuluri P. A Mitochondrial Complex 1 protein encoded by orf19.2500 is critical for Candida albicans growth on alternative carbon sources, immune evasion and virulence. GRC Immunology of Fungal Infections. 2019 Jan 13-18; Galveston, TX.

2018

20. Singh S, Uppuluri P, Alqarihi A, Hennessey JP, Edwards JE, Ibrahim AS. The Candida albicans rAls3p-N vaccine (NDV-3A) protects neutropenic mice from multi-drug resistant C. auris infection. 14th ASM Conference on Candida and Candidiasis; 2018 April 15; Providence, Rhode Island, USA.

21. Alqarihi A, Singh S, Edwards JE, Hennessy JP, Ibrahim AS, Uppuluri P. Murine antibodies induced by an NDV-3A vaccine containing rAls3p-N, abrogate Candida albicans biofilm formation. 14th ASM Conference on Candida and Candidiasis; 2018 April 15; Providence, Rhode Island, USA.

22. Mamouei Z, Singh S, Alqarihi A, Zeng G, Wang Y, Edwards JE, Ibrahim AS, Uppuluri P. Dispersal cells from glucose-replete C. albicans biofilms express a pre-adaptive response of carbon starvation: role of a novel gene orf19.2500. 14th ASM Conference on Candida and Candidiasis; 2018 April 15; Providence, Rhode Island, USA.

23. Felber, Barbara, Shakti Singh, Antonio Valentin, Margherita Rosati, Eric Ramierz, Rami Doueiri, Xintao Hu, et al. “DNA and protein co-delivery vaccines using TLR-4-based adjuvants induce potent immune responses able to delay heterologous SIV acquisition.” In Journal of Medical Primatology, 47:5, 313–313. WILEY, Sept 2018.

2017

24. Felber B, Singh S, Valentin A, Rosati M, Ramierz E, Doueiri R, Hu X, Bear J, Hirsch V, Broderick K, Sardesai N, Reed S, Trinh H, Rao M, Montefiori D, Ferrari G, Shen X, Tomaras G, Pavlakis G. DNA and protein co‐delivery vaccines using TLR‐ 4‐based adjuvants induce potent immune responses able to delay heterologous SIV acquisition. 35th Annual Symposium on Nonhuman Primate Models for AIDS; 2017, Aug 22-25; Madison, Wisconsin, USA.

25. Pavlakis G, Bergamaschi C, Ng SSM, Valentin A, Watson D, Nagy B, Hu X, Alicea C, Jensen S, Fox B, Moysi E, Petrovas C, Singh S, Bear J, and Felber B. Development of heterodimeric IL-15 for cancer immunotherapy. Sep 16-21, 2017; International Conference on Human & Translational Immunology. Rhodes, Greece.

2014

26. Singh S, Li W, Kumar R, Agrawal B. Fine-tuning of host immune responses: A tale of adeno-vector expressing hepatitis C virus antigen NS4. 8th Vaccine & ISV Congress. Oct 26-28, 2014;  Philadelphia, PA, USA.

27. Singh S, Vedi S, Li W, Samrat SK, Kumar R, Agrawal B. Fine-tuning of host immune responses: A tale of adeno-vector expressing hepatitis C virus antigen NS4. 3rd International Conference and Exhibition on Clinical & Cellular Immunology. Sep 29 - Oct 01, 2014; Baltimore, USA.

28. Singh S, Vedi S, Li W, Samrat SK, Kumar R, Agrawal B. Recombinant adenoviral vector expressing HCV NS4 induces protective immune responses in a mouse model of Vaccinia-HCV virus infection: A dose and route conundrum. 21st International Symposium on Hepatitis C and Related Viruses. Sep 7-11, 2014; Banff, AB, Canada.

29. Singh S, Li W, Kumar R, Agrawal B. Protective immunity against Vaccinia-HCV in a mouse model depends upon route and dose of immunization. 27th CIHR-CSHRF (CIHR-Canadian Student Health Research Forum) Symposium. June 10-12, 2014; Winnipeg, MB,Canada.

2012

30. Singh S, Li W, Tyrrell LJ, Kumar R, Agrawal B. Antiviral immune responses against adenovirus expressing HCV NS4 protein depend upon route and dose of immunization. Immunet Retreat. June 21, 2012; Edmonton, Alberta, Canada.

2011

31. Singh S, Li W, Kumar R, Tyrrell LJ, Agrawal B. CD4+ T cell responses induced by adenoviral vector expressing HCV NS4 antigen are dependent on route of immunization and suppressed by high antigen doses. Immunet Retreat. June 10-11, 2011; Edmonton, Alberta, Canada.

Books Edited 

• Kumar A, Singh S, eds. (2016).  Influenza Virus Vaccines and Immunotherapies. Lausanne: Frontiers Media SA.  ISSN 1664-8714. ISBN 978-2-88919-811-5. [DOI: 10.3389/978-2-88919-811-5].

• Agrawal B, Yanow S, Singh S, eds. (2020). Heterologous Immunity: Implications and Applications in Vaccines and Immunotherapies. Lausanne: Frontiers Media SA. ISSN 1664-8714. ISBN 978-2-88963-965-6. [DOI: 10.3389/978-2-88963-965-6]

Book Chapters

1. Lionakis MS, Singh S, Ibrahim AS, Edwards JE (2022). Chapter: 216. Candidiasis. In Dennis L. Kasper and Anthony S. Fauci (eds.) Harrison's Principal’s of Internal Medicine. McGraw Hill Publishers, 21st Edition, ISBN: 978-1-264-26846-7.

2. Singh S, Ibrahim AS, Edwards JE (2019).  Candida auris: An emerging multi-drug resistant Candida species.  In J. Larry Jameson, Anthony S. Fauci, Dennis L. Kasper, Stephen L. Hauser, Dan L. Longo, Joseph Loscalzo (eds.) Harrison's Principles of Internal Medicine, 20th Edition. McGraw Hill Publishers.

3. Singh S, Kumar R, and Agrawal B (2018). Future of Adenoviruses as Vaccine Vector. In Yulia A Desheva (eds.) Adenoviruses, London, United Kingdom: Intech Publishers. [DOI: 10.5772/intechopen.79697].

4. Sarkar A, Kundu S, Singh S, and Sen S (2018). Data Mining in Nutrigenomics. In Shampa Sen, Leonid Datta and Sayak Mitra, eds. Machine Learning and IoT: A Biological Perspective. (pp 40-53). New York, USA: CRC Press, Taylor & Francis Group. ISBN 9781138492691. 

Patents

1. Singh S, Edwards J, Uppuluri P, Ibrahim AS. Methods of Treatment for Candida auris Infections. PCT/US2018/026889, EP18166876.5; Publication Date: Oct 16, 2019. [Link]

2. Singh S, Kumar R. & Agrawal B. Methods of Inducing Immune Response to Hepatitis C Virus. US patent application No. 62/103919; PCT Application No. CA2016/000015; Publication No. WO/2016/112459, Publication Date: July 21, 2016. [Link]